diff --git a/.changeset/brown-grapes-fry.md b/.changeset/brown-grapes-fry.md
new file mode 100644
index 0000000000..c2fd1fbe2a
--- /dev/null
+++ b/.changeset/brown-grapes-fry.md
@@ -0,0 +1,7 @@
+---
+'@backstage/ui': patch
+---
+
+Added indeterminate state support to the Checkbox component for handling partial selection scenarios like table header checkboxes.
+
+Affected components: Checkbox
diff --git a/packages/ui/report.api.md b/packages/ui/report.api.md
index ca9d2bcff1..fa50111cde 100644
--- a/packages/ui/report.api.md
+++ b/packages/ui/report.api.md
@@ -460,6 +460,7 @@ export const CheckboxDefinition: {
   };
   readonly dataAttributes: {
     readonly selected: readonly [true, false];
+    readonly indeterminate: readonly [true, false];
   };
 };
 
diff --git a/packages/ui/src/components/Checkbox/Checkbox.module.css b/packages/ui/src/components/Checkbox/Checkbox.module.css
index 23a88d2b80..a4647c935a 100644
--- a/packages/ui/src/components/Checkbox/Checkbox.module.css
+++ b/packages/ui/src/components/Checkbox/Checkbox.module.css
@@ -62,7 +62,14 @@
       color: var(--bui-fg-solid);
     }
 
-    .bui-Checkbox[data-hovered]:not([data-selected]) & {
+    .bui-Checkbox[data-indeterminate] & {
+      background-color: var(--bui-bg-surface-1);
+      box-shadow: inset 0 0 0 1px var(--bui-border);
+      color: var(--bui-fg-primary);
+    }
+
+    .bui-Checkbox[data-hovered]:not([data-selected]):not([data-indeterminate])
+      & {
       box-shadow: inset 0 0 0 1px var(--bui-border-hover);
     }
 
diff --git a/packages/ui/src/components/Checkbox/Checkbox.stories.tsx b/packages/ui/src/components/Checkbox/Checkbox.stories.tsx
index c5318cc6fb..e8f7859785 100644
--- a/packages/ui/src/components/Checkbox/Checkbox.stories.tsx
+++ b/packages/ui/src/components/Checkbox/Checkbox.stories.tsx
@@ -28,16 +28,27 @@ export const Default = meta.story({
   },
 });
 
+export const Indeterminate = meta.story({
+  args: {
+    children: 'Select all',
+    isIndeterminate: true,
+  },
+});
+
 export const AllVariants = meta.story({
   ...Default.input,
   render: () => (
     <Flex direction="column" gap="2">
       <Checkbox>Unchecked</Checkbox>
       <Checkbox isSelected>Checked</Checkbox>
+      <Checkbox isIndeterminate>Indeterminate</Checkbox>
       <Checkbox isDisabled>Disabled</Checkbox>
       <Checkbox isSelected isDisabled>
         Checked & Disabled
       </Checkbox>
+      <Checkbox isIndeterminate isDisabled>
+        Indeterminate & Disabled
+      </Checkbox>
     </Flex>
   ),
 });
diff --git a/packages/ui/src/components/Checkbox/Checkbox.tsx b/packages/ui/src/components/Checkbox/Checkbox.tsx
index 97a603daf2..6030fbc06b 100644
--- a/packages/ui/src/components/Checkbox/Checkbox.tsx
+++ b/packages/ui/src/components/Checkbox/Checkbox.tsx
@@ -21,7 +21,7 @@ import { useStyles } from '../../hooks/useStyles';
 import { CheckboxDefinition } from './definition';
 import clsx from 'clsx';
 import styles from './Checkbox.module.css';
-import { RiCheckLine } from '@remixicon/react';
+import { RiCheckLine, RiSubtractLine } from '@remixicon/react';
 
 /** @public */
 export const Checkbox = forwardRef<HTMLLabelElement, CheckboxProps>(
@@ -35,12 +35,23 @@ export const Checkbox = forwardRef<HTMLLabelElement, CheckboxProps>(
         className={clsx(classNames.root, styles[classNames.root], className)}
         {...rest}
       >
-        <div
-          className={clsx(classNames.indicator, styles[classNames.indicator])}
-        >
-          <RiCheckLine size={12} />
-        </div>
-        {children}
+        {({ isIndeterminate }) => (
+          <>
+            <div
+              className={clsx(
+                classNames.indicator,
+                styles[classNames.indicator],
+              )}
+            >
+              {isIndeterminate ? (
+                <RiSubtractLine size={12} />
+              ) : (
+                <RiCheckLine size={12} />
+              )}
+            </div>
+            {children}
+          </>
+        )}
       </RACheckbox>
     );
   },
diff --git a/packages/ui/src/components/Checkbox/definition.ts b/packages/ui/src/components/Checkbox/definition.ts
index 022f2a0af1..c7b5b16276 100644
--- a/packages/ui/src/components/Checkbox/definition.ts
+++ b/packages/ui/src/components/Checkbox/definition.ts
@@ -27,5 +27,6 @@ export const CheckboxDefinition = {
   },
   dataAttributes: {
     selected: [true, false] as const,
+    indeterminate: [true, false] as const,
   },
 } as const satisfies ComponentDefinition;